In vitro REGULATION OF THE EXPRESSION OF THE SARS-CoV-2 RECEPTOR ANGIOTENSIN-CONVERTING ENZYME (ACE2) IN LUNG CANCER CELLS BY NATURAL PRODUCTS

The COVID-19 pandemic continues infecting people causing deaths globally. Although various medicines have been tried to combat with COVID-19, there is no medicine or treatment that has validated yet. People using natural products for centuries against bacterial and viral illnesses. This study aimed test the effects of biomolecule oleuropein, whey collected from industrial waste soaproot extracts obtained Gypsophila arrostii Guss. var. nebulosa Boiss. & Heldr. Saponaria officinalis L. on expression human ACE2 gene as SARS-CoV-2 receptor A549 adenocarcinoma cell-line by Real-Time Quantitative Polymerase Chain Reaction (qPCR). According cytotoxicity tests, G. S. extract treatments showed a dose dependent cytotoxic effect cells. EC50 values were found be 54.3 μg/ml 17.3 μg/ml, respectively. Oleuropein moderate value over 250 μg/ml. Whey (fermented non-fermented) did not show any at applied doses. qPCR results mRNA level decreased 89.8% 35.2% due fermented non-fermented extracts, Similarly, downregulated 79.8% 90.1%, In contrast, oleropein upregulated (102.8%). Our supporting produced can used both cancer patients in potential risk groups.